Hemoglobin Alpha Polyclonal antibody

Hemoglobin Alpha Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 14537-1-AP

Synonyms

Alpha globin, HBA1, HBA2, Hemoglobin a, Hemoglobin alpha chain, Hemoglobin subunit alpha, hemoglobin, alpha 1



Tested Applications

Positive WB detected inhuman placenta tissue, mouse heart, mouse liver, rat liver
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

14537-1-AP targets Hemoglobin Alpha in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hemoglobin Alpha fusion protein Ag6040
Full Name hemoglobin, alpha 1
Calculated molecular weight 15 kDa
Observed molecular weight 14-16 kDa, 27 kDa
GenBank accession numberBC050661
Gene symbol HBA1
Gene ID (NCBI) 3039
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.

Protocols

Product Specific Protocols
WB protocol for Hemoglobin Alpha antibody 14537-1-APDownload protocol
IP protocol for Hemoglobin Alpha antibody 14537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Haematologica

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio
mouse

Arch Biochem Biophys

SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes.

Authors - Takujiro Homma
mouseWB

Haematologica

Therapeutic effects of induced pluripotent stem cells in chimeric mice with beta-thalassemia.

Authors - Guanheng Yang
humanWB

Stem Cell Reports

FAM122A Inhibits Erythroid Differentiation through GATA1.

Authors - Jing Chen