Hemoglobin Alpha Polyclonal antibody

• Featured Product
• KD/KO Validated

Hemoglobin Alpha Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 14537-1-AP

Synonyms

Alpha globin, HBA1, HBA2, Hemoglobin a, Hemoglobin Alpha, Hemoglobin alpha chain, Hemoglobin subunit alpha, hemoglobin, alpha 1

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Validation Data Gallery

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  WB analysis using 14537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14537-1-AP

  IP result of anti-Hemoglobin Alpha (IP:14537-1-AP, 4ug; Detection:14537-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IF Staining of human appendicitis using 14537-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Hemoglobin Alpha antibody (14537-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse heart tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IF detected in: human appendicitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14537-1-AP targets Hemoglobin Alpha in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hemoglobin Alpha fusion protein Ag6040
• Full Name: hemoglobin, alpha 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 14-16 kDa, 27 kDa
• GenBank Accession Number: BC050661
• Gene Symbol: HBA1
• Gene ID (NCBI): 3039
• RRID: AB_2114462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
mouse	WB,IHC	Int J Mol Sci; Regulation and Role of Neuron-Derived Hemoglobin in the Mouse Hippocampus.; Authors - Yujiao Lu ; KD Validated
human	WB	Stem Cell Reports; FAM122A Inhibits Erythroid Differentiation through GATA1.; Authors - Jing Chen
mouse	WB	Haematologica; Therapeutic effects of induced pluripotent stem cells in chimeric mice with beta-thalassemia.; Authors - Guanheng Yang
mouse	WB	Nutrients; Cellular Zinc Deficiency Impairs Heme Biosynthesis in Developing Erythroid Progenitors; Authors - Juyoung Kim
mouse		Arch Biochem Biophys; SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes.; Authors - Takujiro Homma