Tested Applications
| Positive WB detected in | human placenta tissue, mouse heart tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IF-P detected in | human appendicitis tissue, human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14537-1-AP targets Hemoglobin Alpha in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6040 Product name: Recombinant human HBA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC050661 Sequence: MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR Predict reactive species |
| Full Name | hemoglobin, alpha 1 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 14-16 kDa, 27 kDa |
| GenBank Accession Number | BC050661 |
| Gene Symbol | HBA1 |
| Gene ID (NCBI) | 3039 |
| RRID | AB_2114462 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P69905 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Hemoglobin Alpha antibody 14537-1-AP | Download protocol |
| IP protocol for Hemoglobin Alpha antibody 14537-1-AP | Download protocol |
| WB protocol for Hemoglobin Alpha antibody 14537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Hemoglobin antibody (Cat# 14537-1-AP) has 17 citations across journals including Circulation, Nature Communications, Journal of Experimental Medicine, Haematologica, eLife, Stem Cell Reports, Brain, Behavior, and Immunity, Molecular Cells, International Journal of Molecular Sciences, European Journal of Pharmacology, Pharmaceutical Biology, Nutrients, Archives of Biochemistry and Biophysics, International Journal of Dentistry, and Journal of Veterinary Medical Science. Research fields span hematology, neuroscience, cardiovascular biology, heme biosynthesis, oncology, immunology, and wound healing.
| Species | Application | Title |
|---|---|---|
Circulation Sickle Trait and Alpha Thalassemia Increase NOS-Dependent Vasodilation of Human Arteries Through Disruption of Endothelial Hemoglobin-eNOS Interactions | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
J Exp Med WDR82-binding long noncoding RNA lncEry controls mouse erythroid differentiation and maturation | ||
Mol Cells Adherent-to-suspension transition modulates circulating tumor cell dynamics and metastatic potential in melanoma. | ||
Haematologica Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. | ||
Haematologica Therapeutic effects of induced pluripotent stem cells in chimeric mice with beta-thalassemia. |
