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- KD/KO Validated
Hemoglobin Alpha Polyclonal antibody
Hemoglobin Alpha Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, ELISA
Cat no : 14537-1-AP
|Positive WB detected in||human placenta tissue, mouse heart, mouse liver, rat liver|
|Positive IP detected in||mouse liver tissue|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 5 publications below|
|IHC||See 1 publications below|
14537-1-AP targets Hemoglobin Alpha in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Hemoglobin Alpha fusion protein Ag6040|
|Full Name||hemoglobin, alpha 1|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||14-16 kDa, 27 kDa|
|GenBank accession number||BC050661|
|Gene ID (NCBI)||3039|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.
|Product Specific Protocols|
|WB protocol for Hemoglobin Alpha antibody 14537-1-AP||Download protocol|
|IP protocol for Hemoglobin Alpha antibody 14537-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cellular Zinc Deficiency Impairs Heme Biosynthesis in Developing Erythroid Progenitors
Stem Cell Reports
FAM122A Inhibits Erythroid Differentiation through GATA1.
Therapeutic effects of induced pluripotent stem cells in chimeric mice with beta-thalassemia.
Arch Biochem Biophys
SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes.
Int J Mol Sci
Regulation and Role of Neuron-Derived Hemoglobin in the Mouse Hippocampus.
Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.