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Hemoglobin Alpha Polyclonal antibody

Hemoglobin Alpha Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14537-1-AP


Alpha globin, HBA1, HBA2, Hemoglobin a, Hemoglobin Alpha, Hemoglobin alpha chain, Hemoglobin subunit alpha, hemoglobin, alpha 1

Tested Applications

Positive WB detected inhuman placenta tissue, mouse heart tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IF detected inhuman appendicitis tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14537-1-AP targets Hemoglobin Alpha in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hemoglobin Alpha fusion protein Ag6040
Full Name hemoglobin, alpha 1
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 14-16 kDa, 27 kDa
GenBank Accession NumberBC050661
Gene Symbol HBA1
Gene ID (NCBI) 3039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.


Product Specific Protocols
WB protocol for Hemoglobin Alpha antibody 14537-1-APDownload protocol
IF protocol for Hemoglobin Alpha antibody 14537-1-APDownload protocol
IP protocol for Hemoglobin Alpha antibody 14537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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