Hemoglobin Alpha Polyclonal antibody, PBS Only

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• Hemoglobin Alpha Polyclonal antibody, PBS Only (14537-1-PBS)

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  WB analysis using 14537-1-AP (same clone as 14537-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14537-1-AP (same clone as 14537-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14537-1-AP (Hemoglobin Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 14537-1-AP (same clone as 14537-1-PBS)

  IP result of anti-Hemoglobin Alpha (IP:14537-1-AP, 4ug; Detection:14537-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 14537-1-AP (same clone as 14537-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Hemoglobin Alpha antibody (14537-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 14537-1-AP (same clone as 14537-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Hemoglobin Alpha antibody (14537-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14537-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Hemoglobin Alpha Polyclonal Antibody for WB, IF-P, IP, ELISA

Cat No. 14537-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA

Synonyms

HBA1, Alpha globin, Alpha-globin, HBA2, Hemoglobin a

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Product Information

14537-1-PBS targets Hemoglobin Alpha in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6040

  Product name: Recombinant human HBA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-142 aa of BC050661

  Sequence: MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR

• Full Name: hemoglobin, alpha 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 14-16 kDa, 27 kDa
• GenBank Accession Number: BC050661
• Gene Symbol: HBA1
• Gene ID (NCBI): 3039
• RRID: AB_2114462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P69905
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.