HBB Polyclonal antibody

HBB Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 16216-1-AP

Synonyms

Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin

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Validation Data Gallery

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  WB analysis of human heart using 16216-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16216-1-AP (HBB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16216-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16216-1-AP (HBB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16216-1-AP

  IP result of anti-HBB (IP:16216-1-AP, 4ug; Detection:16216-1-AP 1:1000) with mouse heart tissue lysate 3200ug.

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  IHC staining of human liver using 16216-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16216-1-AP (HBB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16216-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16216-1-AP (HBB antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of human placenta using 16216-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using HBB antibody (16216-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human appendicitis using 16216-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using HBB antibody (16216-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 16216-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug Anti-Human HBB (16216-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human heart tissue, human placenta tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human appendicitis tissue, human placenta tissue
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 3 publications below

Product Information

16216-1-AP targets HBB in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HBB fusion protein Ag9216
• Full Name: hemoglobin, beta
• Calculated Molecular Weight: 147 aa, 16 kDa
• Observed Molecular Weight: 13-16 kDa
• GenBank Accession Number: BC007075
• Gene Symbol: HBB
• Gene ID (NCBI): 3043
• RRID: AB_10598329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
mouse	WB,IHC	Elife; ARID1A loss in adult hepatocytes activates β-catenin-mediated erythropoietin transcription.; Authors - Rozenn Riou
human	WB	Stem Cells; Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells.; Authors - Amy Leung
		Sci Rep; EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.; Authors - Inn-Inn Chen
mouse	IHC	J Comp Neurol; Hemovasculogenic origin of blood vessels in the developing mouse brain.; Authors - Miguel A Gama Sosa
human	WB	Blood Cells Mol Dis; A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression.; Authors - Tasha A Morrison

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