HECTD1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84918-5-RR (same clone as 84918-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84918-5-RR (HECTD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84918-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84918-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84918-5-RR against Human HECTD1 were performed. The affinity constant is 4.51 nM.

HECTD1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84918-5-PBS

Clone No.242344A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

HECT domain containing 1, EULIR, EC:2.3.2.26, E3 ubiquitin-protein ligase HECTD1, E3 ligase for inhibin receptor

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Product Information

84918-5-PBS targets HECTD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14581

  Product name: Recombinant human HECTD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 29-121 aa of BC006237

  Sequence: PGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

• Full Name: HECT domain containing 1
• Calculated Molecular Weight: 2612 aa, 290 kDa
• Observed Molecular Weight: 290 kDa
• GenBank Accession Number: BC006237
• Gene Symbol: HECTD1
• Gene ID (NCBI): 25831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULT8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This antibody is specific to HECTD1.