HECW2 Polyclonal antibody, PBS Only

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• HECW2 Polyclonal antibody, PBS Only (32026-1-PBS)

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  WB analysis using 32026-1-AP (same clone as 32026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32026-1-AP (HECW2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32026-1-PBS in a different storage buffer formulation.

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  WB analysis using 32026-1-AP (same clone as 32026-1-PBS)

  A549 cells lysate was subjected to SDS PAGE followed by western blot with 32026-1-AP (HECW2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32026-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 32026-1-AP (same clone as 32026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32026-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 32026-1-AP (same clone as 32026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32026-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 32026-1-AP (same clone as 32026-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HECW2 antibody (32026-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 32026-1-PBS in a different storage buffer formulation.

HECW2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 32026-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase HECW2, EC:2.3.2.26, HECT, C2 and WW domain-containing protein 2, HECT-type E3 ubiquitin transferase HECW2, KIAA1301

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Product Information

32026-1-PBS targets HECW2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36316

  Product name: Recombinant human HECW2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 450-650 aa of XM_017004563.1

  Sequence: SFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPEPQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVT

• Full Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
• Calculated Molecular Weight: 176 kDa，1572aa
• Observed Molecular Weight: 240 kDa
• GenBank Accession Number: XM_017004563.1
• Gene Symbol: HECW2
• Gene ID (NCBI): 57520
• RRID: AB_3742511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9P2P5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HECW2, also known as NEDL2 (Nedd4-like E3 ubiquitin-protein ligase 2), is a member of the HECT (Homologous to the E6-AP Carboxyl Terminus) family of E3 ubiquitin ligases. It is crucial in various pathophysiological processes, including cell proliferation, apoptosis, autophagy, immune response, and DNA damage repair (PMID: 39529070). HECW2 is known to stabilize and enhance the transcriptional activity of p73, a member of the p53 family of tumor suppressors, which promotes cell cycle arrest and apoptosis and has an essential role in neurodevelopment (PMID: 27389779). In cancer, HECW2 is a promising target for tumor therapy due to its role in regulating cell cycle progression and angiogenesis (PMID: 39529070). In neurodevelopmental disorders, variants in HECW2 have been reported to cause a disorder characterized by hypotonia, seizures, and impaired language (PMID: 34321324).