CoraLite® Plus 488-conjugated HLA-DMB Recombinant antibody

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  IF Staining of Raji using CL488-82922

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 488 HLA-DMB antibody (CL488-82922, Clone: 230123A3 ) at dilution of 1:200.

HLA-DMB Recombinant Antibody for IF/ICC

Cat No. CL488-82922

Clone No.230123A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

230123A3, DMB, HLA DMB, MHC class II antigen DMB, RING7

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Tested Applications

• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82922 targets HLA-DMB in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HLA-DMB fusion protein Ag16320
• Full Name: major histocompatibility complex, class II, DM beta
• Calculated Molecular Weight: 263 aa, 29 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC027175
• Gene Symbol: HLA-DMB
• Gene ID (NCBI): 3109
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28068
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.