Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 65560-1-MR (same clone as 65560-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65560-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65560-1-MR (same clone as 65560-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243)(red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65560-1-PBS in a different storage buffer formulation.

HLA-DR Uni-rAb^® Recombinant Antibody for FC

Cat No. 65560-1-PBS

Clone No.L243

Host / Isotype

Mouse / IgG2a

Reactivity

human, monkey, non-human primates

Applications

FC

Synonyms

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

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Product Information

65560-1-PBS targets HLA-DR in FC applications and shows reactivity with human, monkey, non-human primates samples.

• Tested Reactivity: human, monkey, non-human primates
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human lymphoblastoid B-cell line RPMI 8866

• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• ENSEMBL Gene ID: ENSG00000204287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P01903
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

HLA-DR is a member of MHC class II molecules encoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participates in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).