HOXB6 Recombinant monoclonal antibody

• 

  IF Staining of HepG2 using 83155-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOXB6 antibody (83155-4-RR, Clone: 230433F10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  IF Staining of A431 using 83155-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HOXB6 antibody (83155-4-RR, Clone: 230433F10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  FC experiment of K562 using 83155-4-RR

  1x10^6 K562 were intracellularly stained with 0.25 ug HOXB6 Recombinant antibody (83155-4-RR, Clone:230433F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 83155-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83155-4-RR against Human HOXB6 were performed. The affinity constant is 0.186 nM.

HOXB6 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83155-4-RR

Clone No.230433F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Hox 2.2, Homeobox protein Hu-2, Homeobox protein Hox-B6, Homeobox protein Hox-2B, Homeobox protein Hox-2.2

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells, A431 cells
• Positive FC (Intra) detected in: K562

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83155-4-RR targets HOXB6 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14189

  Product name: Recombinant human HOXB6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-160 aa of BC014651

  Sequence: MSSYFVNSTFPVTLASGQESFLGQLPLYSSGYADPLRHYPAPYGPGPGQDKGFATSSYYPPAGGGYGRAAPCDYGPAPAFYREKESACALSGADEQPPFHPEPRKSDCAQDKSVFGETEEQKCSTPVYPWMQRMNSCNSSSFGPSGRRGRQTYTRYQTLE

• Full Name: homeobox B6
• Calculated Molecular Weight: 224 aa, 25 kDa
• GenBank Accession Number: BC014651
• Gene Symbol: HOXB6
• Gene ID (NCBI): 3216
• RRID: AB_3670849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P17509
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXB6 is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development, including that of lung and skin, and has been localized to both the nucleus and cytoplasm. Altered expression of this gene or a change in the subcellular localization of its protein is associated with some cases of acute myeloid leukemia and colorectal cancer.