CoraLite® Plus 488-conjugated HOXB6 Recombinant monoclonal antibody

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  IF Staining of HepG2 using CL488-83155-4

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HOXB6 antibody (CL488-83155-4, Clone: 230433F10 ) at dilution of 1:200.

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  FC experiment of K-562 using CL488-83155-4

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated HOXB6 Recombinant antibody (CL488-83155-4, Clone:230433F10)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

HOXB6 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL488-83155-4

Clone No.230433F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Hox 2.2, Homeobox protein Hu-2, Homeobox protein Hox-B6, Homeobox protein Hox-2B, Homeobox protein Hox-2.2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83155-4 targets HOXB6 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14189

  Product name: Recombinant human HOXB6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-160 aa of BC014651

  Sequence: MSSYFVNSTFPVTLASGQESFLGQLPLYSSGYADPLRHYPAPYGPGPGQDKGFATSSYYPPAGGGYGRAAPCDYGPAPAFYREKESACALSGADEQPPFHPEPRKSDCAQDKSVFGETEEQKCSTPVYPWMQRMNSCNSSSFGPSGRRGRQTYTRYQTLE

• Full Name: homeobox B6
• Calculated Molecular Weight: 224 aa, 25 kDa
• GenBank Accession Number: BC014651
• Gene Symbol: HOXB6
• Gene ID (NCBI): 3216
• RRID: AB_3673205
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P17509
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HOXB6 is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development, including that of lung and skin, and has been localized to both the nucleus and cytoplasm. Altered expression of this gene or a change in the subcellular localization of its protein is associated with some cases of acute myeloid leukemia and colorectal cancer.