HRAS-Specific Polyclonal antibody, PBS Only

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• HRAS-Specific Polyclonal antibody, PBS Only (18295-1-PBS)

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  WB analysis using 18295-1-AP (same clone as 18295-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 18295-1-AP (same clone as 18295-1-PBS)

  WB result of HRAS-Specific antibody (18295-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRAS-Specific transfected HEK-293 cells. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

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  WB analysis using 18295-1-AP (same clone as 18295-1-PBS)

  Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 18295-1-AP (same clone as 18295-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 18295-1-AP (same clone as 18295-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 18295-1-AP (same clone as 18295-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18295-1-AP (same clone as 18295-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 18295-1-AP (same clone as 18295-1-PBS)

  IP result of anti-HRAS-Specific (IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 18295-1-AP (same clone as 18295-1-PBS)

  IP result of anti-HRAS-Specific (IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18295-1-PBS

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 18295-1-AP (HRAS-Specific Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 18295-1-AP (same clone as 18295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18295-1-AP (HRAS-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 18295-1-AP (same clone as 18295-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HRAS-Specific antibody (18295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 18295-1-AP (same clone as 18295-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HRAS-Specific (18295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HRAS-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18295-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

GTPase HRas, H RASIDX, HRAS, HRAS1, EC:3.6.5.2

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Product Information

18295-1-PBS targets HRAS-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-Ha-ras Harvey rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC006499
• Gene Symbol: HRAS
• Gene ID (NCBI): 3265
• RRID: AB_2121046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01112
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.