Human B Cell Basics Panel

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  1 x 10^6 Human PBMCs were stained with PK30008 Human B Cell Basics Panel. CD45+ cells are gated. Parent population: lymphocytes. Cells were not fixed.

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  1 x 10^6 Human PBMCs were stained with PK30008 Human B Cell Basics Panel. CD3-/CD19+ cells are gated. Parent population: CD45+ lymphocytes. Cells were not fixed.

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  1 x 10^6 Human PBMCs were stained with PK30008 Human B Cell Basics Panel. CD20 and CD21 expression on CD45+CD3-CD19+ lymphocytes are shown. Cells were not fixed.

Cat No. PK30008

Host

Mouse

Reactivity

Human

Applications

FC

Conjugate

Various

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Product Information

B lymphocytes are immune cells that produce antibodies to neutralize pathogens and foreign substances.

This panel is designed for the investigation of B lymphocytes (CD19 positive) and for the initial separation of the B lymphocyte population into further subpopulations by developmental stage. The PE channel is left open. 

Panel Components

Fluorochrome (ex/em)	Catalog No	Target	Clone	Isotype	Description
FITC Plus	FITC-65109	CD45	HI30	Mouse IgG1k	Lymphocyte marker
(495 nm/524 nm)
CoraLite Plus 750	CL750-65151	CD3	UCHT1	Mouse IgG1k	T cell marker
(755 nm/780 nm)
CoraLite Plus 647	CL647-65197	CD19	4G7	Mouse IgG1k	B cell marker
(654 nm/674 nm)
Cardinal Red	CR-65085	CD20	2H7	Mouse IgG2bk	B cell marker
(592 nm/611 nm)
Atlantic Blue	AB-65198	CD21	BU32	Mouse IgG1k	Expression varies depending on B cell development stage
(404 nm/458 nm)

Storage

Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.

Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 

Usage

Human B Cell Basics Panel Datasheet and User Guide

Flow-Cytometry-Cell-Surface-Staining-Protocol

Cited in Article as

PK30008, Human B Cell Basics Panel, Proteintech, IL, USA