Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 84764-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of Human Hepatocellular cancer using 84764-3-RR
Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84764-3-RR (IARS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84764-3-RR (IARS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of Human Hepatocellular cancer using 84764-3-RR
Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84764-3-RR (IARS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84764-3-RR (IARS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 84764-3-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 84764-3-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of HepG2 using 84764-3-RR
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IARS2 Recombinant antibody (84764-3-RR, Clone:242219C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IARS2 Recombinant antibody (84764-3-RR, Clone:242219C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 84764-3-RR
Biolayer interferometry (BLl) kinetic assays of 84764-3-RR against Human IARS2 were performed. The affinity constant is 0.469 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HeLa cells, mouse lung tissue, HEK-293 cells, MCF-7 cells, Jurkat cells, rat heart tissue
Positive IHC detected in
Human Hepatocellular cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells, HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
84764-3-RR targets IARS2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.
Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 84764-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of Human Hepatocellular cancer using 84764-3-RR
Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84764-3-RR (IARS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of Human Hepatocellular cancer using 84764-3-RR
Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84764-3-RR (IARS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 84764-3-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 84764-3-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC (INTRA) Figures
FC experiment of HepG2 using 84764-3-RR
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IARS2 Recombinant antibody (84764-3-RR, Clone:242219C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 84764-3-RR
Biolayer interferometry (BLl) kinetic assays of 84764-3-RR against Human IARS2 were performed. The affinity constant is 0.469 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
IARS2 Recombinant antibody
Catalog Number
84764-3-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:250-1:1000 IF/ICC : 1:250-1:1000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
