IBA1 Recombinant antibody, PBS Only

IBA1 Recombinant Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D5

Cat no : 81728-1-PBS

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

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Validation Data Gallery

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  WB analysis of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

• 

  FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human IBA1 (81728-1-RR, Clone:4D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81728-1-PBS targets IBA1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IBA1 fusion protein Ag1363
• Full Name: allograft inflammatory factor 1
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC009474
• Gene Symbol: AIF1
• Gene ID (NCBI): 199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.