IBA1 Recombinant antibody

IBA1 Recombinant Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat







Cat no : 81728-1-RR


AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inrat brain tissue, mouse brain tissue, human liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue
Positive FC detected inTHP-1 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81728-1-RR targets IBA1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IBA1 fusion protein Ag1363
Full Name allograft inflammatory factor 1
Calculated Molecular Weight 17 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberBC009474
Gene Symbol AIF1
Gene ID (NCBI) 199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.


Product Specific Protocols
WB protocol for IBA1 antibody 81728-1-RRDownload protocol
IHC protocol for IBA1 antibody 81728-1-RRDownload protocol
IF protocol for IBA1 antibody 81728-1-RRDownload protocol
Standard Protocols
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