FITC-conjugated ICAM-1 Monoclonal antibody

ICAM-1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

FITC Fluorescent Dye

Cat no : FITC-60299

Synonyms

CD54, ICAM1, ICAM-1



Tested Applications

Positive IF detected inhuman lung cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

FITC-60299 targets ICAM-1 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ICAM-1 fusion protein Ag8309
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC015969
Gene symbol ICAM1
Gene ID (NCBI) 3383
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm/520 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% glycerol, with 0.5% BSA, with 0.05% Proclin300.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784). This antibody is FITC conjugated ICAM1 mouse monoclonal antibody (600299-1-Ig) which has passed in WB, IHC and FC detection.

Protocols

Product Specific Protocols
IF protocol for FITC ICAM-1 antibody FITC-60299Download protocol
Standard Protocols
Click here to view our Standard Protocols