Validation Data Gallery
|Positive WB detected in
|HepG2 cells, HeLa cells, DU 145 cells, MCF-7 cells
|Positive IHC detected in
|human pancreas tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:20-1:200
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66197-1-Ig targets IDH1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG1
|IDH1 fusion protein Ag19293
|isocitrate dehydrogenase 1 (NADP+), soluble
|Calculated molecular weight
|414 aa, 47 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.
Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues