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IDH2 Monoclonal antibody
IDH2 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1F10A10
Cat no : 66918-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells, K-562 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Sample-dependent, check data in validation data gallery |
Product Information
66918-1-Ig targets IDH2 in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IDH2 fusion protein Ag8950 |
Full Name | isocitrate dehydrogenase 2 (NADP+), mitochondrial |
Calculated molecular weight | 452 aa, 51 kDa |
Observed molecular weight | 41-47 kDa |
GenBank accession number | BC009244 |
Gene symbol | IDH2 |
Gene ID (NCBI) | 3418 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.
Protocols
Product Specific Protocols | |
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WB protocol for IDH2 antibody 66918-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |