IDO1 Monoclonal antibody

IDO1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G2G11

Cat no : 66528-1-Ig

Synonyms

CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1



Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

66528-1-Ig targets IDO1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDO1 fusion protein Ag3953
Full Name indoleamine 2,3-dioxygenase 1
Calculated molecular weight 403 aa, 45 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC027882
Gene symbol IDO1
Gene ID (NCBI) 3620
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

Protocols

Product Specific Protocols
WB protocol for IDO1 antibody 66528-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Med Rep

Fr‑HMGB1 and ds‑HMGB1 activate the kynurenine pathway via different mechanisms in association with depressive‑like behavior.

Authors - Bo Wang
mouseWB

Molecules

Antidepressant-Like Effect and Mechanism of Action of Honokiol on the Mouse Lipopolysaccharide (LPS) Depression Model.

Authors - Bo Zhang
mouseWB

ACS Chem Neurosci

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Induced Parkinson's Disease in Mouse: Potential Association between Neurotransmitter Disturbance and Gut Microbiota Dysbiosis.

Authors - Yuanhui Zhu
mouseWB

Chin J Integr Med

Combination of Geniposide and Eleutheroside B Exerts Antidepressant-like Effect on Lipopolysaccharide-Induced Depression Mice Model.

Authors - Bo Zhang
humanWB

Elife

PMID: 30132758

Authors - Tonya Kueck