CoraLite® Plus 488-conjugated IDO1 Monoclonal antibody

IDO1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G2G11

Cat no : CL488-66528

Synonyms

CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1



Tested Applications

Positive WB detected inIFN gamma treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66528 targets IDO1 in WB applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDO1 fusion protein Ag3953
Full Name indoleamine 2,3-dioxygenase 1
Calculated Molecular Weight 403 aa, 45 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC027882
Gene Symbol IDO1
Gene ID (NCBI) 3620
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 IDO1 antibody CL488-66528Download protocol
Standard Protocols
Click here to view our Standard Protocols