IFI30 Recombinant antibody

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  WB analysis using 85982-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85982-1-RR (IFI30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IFI30 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85982-1-RR

Clone No.250400A4

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

EC:1.8.-.-, Gamma-interferon-inducible lysosomal thiol reductase, Gamma-interferon-inducible protein IP-30, GILT, IFI 30

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Tested Applications

• Positive WB detected in: HeLa cells, Daudi cells, HL-60 cells, Raji cells, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85982-1-RR targets IFI30 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IFI30 fusion protein Ag2178
• Full Name: interferon, gamma-inducible protein 30
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC021136
• Gene Symbol: IFI30
• Gene ID (NCBI): 10437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13284
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFI30(interferon, gamma-inducible protein 30) is also named as IP30, GILT, IFI-30, MGC32056, IFI30 and belongs to the GILT family. It can facilitate the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. IFI30 is inducibly up-regulated by IFN-g, as are MHC class II molecules, the associated invariant chain, and HLA-DM, which catalyses peptide loading in MIICs and the 35 kDa species is the IFI30 proform, and the 30-kDa species is the mature form(PMID:10639150). It can exsit as a dimer(PMID:17142755).