|Positive WB detected in||HepG2 cells, THP-1 cells, Jurkat cells, Daudi cells, Raji cells|
|Positive IHC detected in||human liver cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
66770-1-Ig targets IFIH1/MDA5 in WB, IHC, ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||IFIH1/MDA5 fusion protein Ag17448|
|Full Name||interferon induced with helicase C domain 1|
|Calculated molecular weight||1025 aa, 117 kDa|
|Observed molecular weight||117-125 kDa|
|GenBank accession number||BC111750|
|Gene ID (NCBI)||64135|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).
Host protein, HSP90β, antagonizes IFN-β signaling pathway and facilitates the proliferation of encephalomyocarditis virus in vitro.
Encephalomyocarditis virus abrogates the IFN-β signaling pathway via its structural protein VP2.