Validation Data Gallery
|Positive WB detected in
|MDA-MB-468 cells, HepG2 cells, THP-1 cells, Jurkat cells, Daudi cells, Raji cells, T-47D cells
|Positive IHC detected in
|human liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:250-1:1000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66770-1-Ig targets IFIH1/MDA5 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Host / Isotype
|Mouse / IgG1
|IFIH1/MDA5 fusion protein Ag17448
|interferon induced with helicase C domain 1
|Calculated molecular weight
|1025 aa, 117 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).
Encephalomyocarditis virus abrogates the IFN-β signaling pathway via its structural protein VP2.
DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation
Host protein, HSP90β, antagonizes IFN-β signaling pathway and facilitates the proliferation of encephalomyocarditis virus in vitro.
Temporally integrated transcriptome analysis reveals ASFV pathology and host response dynamics
SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitination