IFIH1/MDA5 Antibody

IFIH1/MDA5 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D8B11

Cat no : 66770-1-Ig

Synonyms

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, MDA 5, MDA5, RH116



Tested Applications

Positive WB detected inHepG2 cells, THP-1 cells, Jurkat cells, Daudi cells, Raji cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66770-1-Ig targets IFIH1/MDA5 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFIH1/MDA5 fusion protein Ag17448
Full Name interferon induced with helicase C domain 1
Calculated molecular weight 1025aa,117 kDa
Observed molecular weight 117-125 kDa
GenBank accession number BC111750
Gene symbol IFIH1
Gene ID (NCBI) 64135
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).

Protocols

Product Specific Protocols
WB protocol for IFIH1/MDA5 antibody 66770-1-IgDownload protocol
IHC protocol for IFIH1/MDA5 antibody 66770-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols