PerCP Anti-Mouse IFN-beta Rabbit Recombinant Antibody

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  FC experiment of C57BL/6 mouse splenocytes using CP-98082

  1x10^6 C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug PerCP Anti-Mouse IFN-beta Rabbit RecAb (CP-98082, Clone:240230B6)(red) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IFN-beta Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. CP-98082

Clone No.240230B6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Ifnb1, 240230B6, Ifb, IFN beta, IFNB

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Tested Applications

• Positive FC (Intra) detected in: C57BL/6 mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CP-98082 targets IFN-beta in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IFN-beta fusion protein Eg0660
• Full Name: interferon beta 1, fibroblast
• Calculated Molecular Weight: 22KD
• GenBank Accession Number: NM_010510.1
• Gene Symbol: Ifnb1
• Gene ID (NCBI): 15977
• Conjugate: PerCP Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 482 nm / 677 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01575
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interferon-beta (IFN-β) is a type I interferon, which is a group of signaling proteins made and released by host cells in response to the presence of several viruses, including double-stranded RNA produced by many viruses. IFN-β is a single protein species and is molecularly related to IFN-α subtypes, although it is antigenically distinct from them. It is mainly produced by fibroblasts and plays a crucial role in the innate immune response against viruses. IFN-β can inhibit viral replication by interfering with the virus's RNA or DNA, thus suppressing viral growth. It also enhances the cytotoxic activity of natural killer (NK) cells and promotes the expression of major histocompatibility complex (MHC) class I molecules.