CoraLite® Plus 488-conjugated IGF1B-Specific Polyclonal antibody

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  IF Staining of HepG2 using CL488-20215

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 IGF1B-Specific antibody (CL488-20215) at dilution of 1:200.

• Featured Product
• KD/KO Validated

IGF1B-Specific Polyclonal Antibody for IF/ICC

Cat No. CL488-20215

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

IGF1, IGF-1, IGF I, IGFI, IGF-I

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-20215 targets IGF1B-Specific in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: insulin-like growth factor 1 (somatomedin C)
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: NM_001111285
• Gene Symbol: IGF1
• Gene ID (NCBI): 3479
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05019
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IGF1B, also named as IBP1, MGF, IGF-I and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1B.