IGFBP3 Monoclonal antibody

IGFBP3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgM









Cat no : 68402-1-Ig


BP 53, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, IGFBP3

Tested Applications

Positive WB detected inA549 cells, human placenta tissue, Caco-2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68402-1-Ig targets IGFBP3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen IGFBP3 fusion protein Ag20351
Full Name insulin-like growth factor binding protein 3
Calculated Molecular Weight 32 kDa
Observed Molecular Weight39-45 kDa
GenBank Accession NumberBC000013
Gene Symbol IGFBP3
Gene ID (NCBI) 3486
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)


Product Specific Protocols
WB protocol for IGFBP3 antibody 68402-1-IgDownload protocol
IHC protocol for IGFBP3 antibody 68402-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols