IgD Monoclonal antibody

human IgD Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1D1B12

Cat no : 67538-1-Ig

Synonyms

FLJ00382, FLJ46727, IGHD

---

Validation Data Gallery

• 

  WB analysis of human plasma using 67538-1-Ig

  human plasma (0.1 ul) were subjected to SDS PAGE followed by western blot with 67538-1-Ig (human IgD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 67538-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67538-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67538-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 67538-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IGHD antibody (67538-1-Ig, Clone: 1D1B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:10000-1:40000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

67538-1-Ig targets human IgD in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: human IgD fusion protein Ag9614
• Full Name: immunoglobulin heavy constant delta
• Calculated Molecular Weight: 573 aa, 63 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC021276
• Gene Symbol: IGHD
• Gene ID (NCBI): 3495
• RRID: AB_2882757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .

Publications

Species	Application	Title
human	IF	Exp Mol Med; Delineating the dynamic evolution from preneoplasia to invasive lung adenocarcinoma by integrating single-cell RNA sequencing and spatial transcriptomics; Authors - Jianfei Zhu