CoraLite®594-conjugated IgD Monoclonal antibody

human IgD Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D1B12

Cat no : CL594-67538

Synonyms

FLJ00382, FLJ46727, IGHD

---

Validation Data Gallery

• 

  IF Staining of human tonsillitis using CL594-67538

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 human IgD antibody (CL594-67538, Clone: 1D1B12 ) at dilution of 1:100.

---

Tested Applications

• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67538 targets human IgD in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: human IgD fusion protein Ag9614
• Full Name: immunoglobulin heavy constant delta
• Calculated Molecular Weight: 573 aa, 63 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC021276
• Gene Symbol: IGHD
• Gene ID (NCBI): 3495
• RRID: AB_2920154
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .