Tested Applications
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67538 targets IGHD in IF-P applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9614 Product name: Recombinant human IGHD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 202-548 aa of BC021276 Sequence: RRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPGSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYLAMTPLIPQSKDENSDDYTTFDDVGSLWT Predict reactive species |
| Full Name | immunoglobulin heavy constant delta |
| Calculated Molecular Weight | 573 aa, 63 kDa |
| Observed Molecular Weight | 55-70 kDa |
| GenBank Accession Number | BC021276 |
| Gene Symbol | IGHD |
| Gene ID (NCBI) | 3495 |
| RRID | AB_2919496 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 IGHD antibody CL488-67538 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CoraLite® Plus 488-conjugated human IgD Monoclonal antibody (CL488-67538) has 1 total citation. It appears in Nature Communications (2025), in a study comparing T cell and B cell subpopulations in children with adenoid and tonsil hypertrophy, using immunofluorescence (IF) on human samples. The associated research field is immunology, specifically lymphocyte subpopulation analysis in pediatric hypertrophy.
| Species | Application | Title |
|---|---|---|
Nat Commun Parallel comparison of T cell and B cell subpopulations of adenoid hypertrophy and tonsil hypertrophy of children |
