FcZero-rAb® Biotin Anti-Human IL-1 beta Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human IL-1 beta Rabbit Recombinant Antibody (Biotin-FcA98142)

IL-1 beta FcZero-rAb^® Recombinant Antibody for FC (Intra)

Cat No. Biotin-FcA98142

Clone No.240902A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta

• 

  FC experiment of human PBMCs using Biotin-FcA98142

  1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Biotin Anti-Human IL-1 beta Rabbit RecAb (Biotin-FcA98142, Clone: 240902A3) and Streptavidin-PE Conjugate (PE-PF00030). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). Monocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using Biotin-FcA98142

  1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Biotin Anti-Human IL-1 beta Rabbit RecAb (Biotin-FcA98142, Clone: 240902A3) (red) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were intracellularly stained with 5 ul Biotin Anti-Human IL-1 beta Rabbit RecAb (Biotin-FcA98142, Clone: 240902A3) and Streptavidin-PE Conjugate (PE-PF00030) (black, dashed). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). Monocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: IFN gamma, LPS and Brefeldin A treated human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98142 targets IL-1 beta in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0286

  Product name: Recombinant Human IL-1 Beta protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: N-6*HIS

  Domain: 117-269 aa of BC008678

  Sequence: APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

• Full Name: interleukin 1, beta
• Calculated Molecular Weight: 269 aa, 31 kDa
• GenBank Accession Number: BC008678
• Gene Symbol: IL1B
• Gene ID (NCBI): 3553
• ENSEMBL Gene ID: ENSG00000125538
• RRID: AB_3746939
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01584
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.