CoraLite® Plus 488-conjugated IL-1 beta Monoclonal antibody

IL-1 beta Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A1B4

Cat no : CL488-66737

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta



Tested Applications

Positive FC detected inLPS treated THP-1 cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-66737 targets IL-1 beta in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1 beta fusion protein Ag26030
Full Name interleukin 1, beta
Calculated molecular weight 269 aa, 31 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IL-1 beta antibody CL488-66737Download protocol
Standard Protocols
Click here to view our Standard Protocols