CoraLite® Plus 488-conjugated IL-1 beta Monoclonal antibody

IL-1 beta Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A1B4

Cat no : CL488-66737

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

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Validation Data Gallery

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  FC experiment of THP-1 using CL488-66737

  1X10^6 THP-1 cells untreated (dashed lines) or treated with LPS (red) were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-1 beta (CL488-66737, Clone:2A1B4) (red), or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: LPS treated THP-1 cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-66737 targets IL-1 beta in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-1 beta fusion protein Ag26030
• Full Name: interleukin 1, beta
• Calculated molecular weight: 269 aa, 31 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.