Anti-Mouse IL-13 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of C57BL/6 Th2-polarized splenocytes using 98003-1-PBS

  1x10^6 unstimulated or PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-13 Rabbit Recombinant Antibody, PBS Only (98003-1-PBS, Clone:2C14) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IL-13 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98003-1-PBS

Clone No.2C14

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

FC (Intra)

Synonyms

Il13, IL-13, interleukin 13, Interleukin-13, 2C14, Il 13

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Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes
• Positive FC detected in: PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98003-1-PBS targets IL-13 in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 13
• Calculated Molecular Weight: 14 kDa
• GenBank Accession Number: NM_008355
• Gene Symbol: IL-13
• Gene ID (NCBI): 16163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20109
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

Interleukin 13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. IL-13 up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. IL-13 is thought to be important cytokine in the pathogenesis of asthma, and more recently has been shown to play a pivotal role in a number of fibrotic diseases including hepatic and pulmonary fibrosis, and nodular sclerosing Hodgkin's disease.