Anti-Human IL-17RA Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using 98271-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16 (3G8). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98271-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98271-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98271-1-RR against Human IL-17RA/CD217 were performed. The affinity constant is 0.115 nM.

IL-17RA Uni-rAb^® Recombinant Antibody for FC

Cat No. 98271-1-RR

Clone No.241891G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD217, IL17RA, 241891G7, CANDF5, CDw217

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98271-1-RR targets IL-17RA in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2317

  Product name: Recombinant Human IL-17RA protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 33-320 aa of BC011624

  Sequence: LRLLDHRALVCSQPGLNCTVKNSTCLDDSWIHPRNLTPSSPKDLQIQLHFAHTQQGDLFPVAHIEWTLQTDASILYLEGAELSVLQLNTNERLCVRFEFLSKLRHHHRRWRFTFSHFVVDPDQEYEVTVHHLPKPIPDGDPNHQSKNFLVPDCEHARMKVTTPCMSSGSLWDPNITVETLEAHQLRVSFTLWNESTHYQILLTSFPHMENHSCFEHMHHIPAPRPEEFHQRSNVTLTLRNLKGCCRHQVQIQPFFSSCLNDCLRHSATVSCPEMPDTPEPIPDYMPLW

• Full Name: interleukin 17 receptor A
• Calculated Molecular Weight: 866 aa, 96 kDa
• GenBank Accession Number: BC011624
• Gene Symbol: IL17RA
• Gene ID (NCBI): 23765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96F46
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-17 is a proinflammatory cytokine. IL-17 can signal as homodimers or heterodimers through a heterodimeric receptor formed by IL-17 receptor A (IL-17RA) and IL-17 receptor C (IL-17RC) subunits. IL-17RA is ubiquitously expressed in both the epithelial and hematopoietic cell compartments. IL-17/IL-17RA signaling plays an important physiological role in regulating the mucosal host defense against invading microorganisms, but it can also play pathogenic roles in several inflammatory or autoimmune conditions; furthermore, it has been described as pro-tumorigenic in several cancer types.