CoraLite® Plus 647 Anti-Human IL-2 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL647-98053

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Human IL-2 Rabbit RecAb (CL647-98053, Clone: 240416E3) and FITC Plus Anti-Human CD3 (OKT3) (FITC-65133, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IL-2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. CL647-98053

Clone No.240416E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

IL2, 240416E3, IL 2, interleukin 2, Interleukin-2

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Tested Applications

• Positive FC (Intra) detected in: PMA, Ionomycin and Brefeldin A treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98053 targets IL-2 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-2 fusion protein Eg0828
• Full Name: interleukin 2
• Calculated Molecular Weight: 153 aa, 18 kDa
• GenBank Accession Number: BC070338
• Gene Symbol: IL2
• Gene ID (NCBI): 3558
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P60568
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).