FcZero-rAb® CoraLite® Plus 647 Anti-Human IL-4 Rabbit Recombinant Antibody

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• FcZero-rAb® CoraLite® Plus 647 Anti-Human IL-4 Rabbit Recombinant Antibody (CL647-FcA98138)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

IL-4 FcZero-rAb^® Recombinant Antibody for FC

Cat No. CL647-FcA98138

Clone No.240794E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IL4, B-cell stimulatory factor 1, BSF-1, IL 4, interleukin 4

• 

  FC experiment of human PBMCs using CL647-FcA98138

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with FcZero-rAb® CoraLite® Plus 405 Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human IL-4 Rabbit RecAb (CL647-FcA98138, Clone: 240794E11). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: PMA and ionomycin treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-FcA98138 targets IL-4 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0104

  Product name: Recombinant Human IL-4 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 25-153 aa of BC070123

  Sequence: HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

• Full Name: interleukin 4
• Calculated Molecular Weight: 153 aa, 17 kDa
• GenBank Accession Number: BC070123
• Gene Symbol: IL-4
• Gene ID (NCBI): 3565
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05112
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.