Product Information
84414-6-PBS targets IL-4RA/CD124 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1684 Product name: Recombinant Human IL-4 R alpha/CD124 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 26-232 aa of NM_000418.4 Sequence: MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQH Predict reactive species |
| Full Name | interleukin 4 receptor |
| Calculated Molecular Weight | 90 kDa |
| GenBank Accession Number | NM_000418.4 |
| Gene Symbol | IL-4R |
| Gene ID (NCBI) | 3566 |
| RRID | AB_3671943 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P24394-1 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
Preparation of Suspension Cell Slides 1. Cell collection: Collect the suspension cell suspension, centrifuge at 1200 rpm for 5 min, then discard the supernatant. 2. Cell washing: Resuspend cells with PBS, centrifuge at 3000 rpm for 1 min, and remove the supernatant. Repeat the washing twice to completely remove residual culture medium. Fixation treatment: Resuspend cells with 4% paraformaldehyde, fix at room temperature for 10-15 min, centrifuge at 3000 rpm for 1 min, then discard the supernatant. 3. Fixative washing: Resuspend cells with PBS, centrifuge at 3000 rpm for 1 min, and discard the supernatant. Repeat the washing twice. 4. Coverslip seeding: Resuspend cells with an appropriate amount of PBS. Add 30 μL of cell suspension per well onto the pre-placed coverslips in a 24-well plate. 5. Drying: Place the plate in an oven at approximately 37 °C and dry for 1.5-2 h. The samples can then be used for subsequent tests such as immunofluorescence assay.
