IL-17RA Recombinant antibody, PBS Only (Detector)

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  WB analysis using 84535-2-RR (same clone as 84535-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84535-2-RR (IL17RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84535-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01397-3

  Sandwich ELISA standard curve of MP01397-3, Human IL17RA Recombinant Matched Antibody Pair - PBS only. 84535-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2317. 84535-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP01397-3

  The mean IL17RA concentration was determined to be 8.86 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP01397-1

  Cytometric bead array standard curve of MP01397-1, IL17RA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84535-1-PBS. Detection antibody: 84535-2-PBS. Standard: Eg2317. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84535-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84535-2-RR against Human IL17RA were performed. The affinity constant is below 1 pM.

IL-17RA Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84535-2-PBS

Clone No.241891E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD217, IL17RA, 241891E11, CANDF5, CDw217

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Product Information

84535-2-PBS targets IL-17RA as part of a matched antibody pair:

MP01397-1: 84535-1-PBS capture and 84535-2-PBS detection (validated in Cytometric bead array)

MP01397-3: 84535-3-PBS capture and 84535-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-17RA fusion protein Eg2317
• Full Name: interleukin 17 receptor A
• Calculated Molecular Weight: 866 aa, 96 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC011624
• Gene Symbol: IL17RA
• Gene ID (NCBI): 23765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96F46
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-17 receptor A (IL17A) is a major effector cytokine of the innate and adaptive immune system and is involved in antimicrobial host defense and maintenance of tissue integrity. It has been shown that IL-17A is involved in the pathogenesis of asthma and allergy and regulates local immune and inflammatory processes in bone tissue and is involved in the onset and progression of osteoporosis. In addition to this, due to polymorphisms in the IL17RA gene, it leads to the development of Fanconi anemia.