IL-4RA Monoclonal antibody

IL-4RA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E4C11

Cat no : 67051-1-Ig

Synonyms

IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein

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Validation Data Gallery

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  WB analysis using 67051-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MG-63 using 67051-1-Ig

  MG-63 cells were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67051-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL4R antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, MG-63 cells, SCaBER cells, HT-1376 cells, Raji cells, Ramos cells, Daudi cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67051-1-Ig targets IL-4RA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-4RA fusion protein Ag28411
• Full Name: interleukin 4 receptor
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_000418
• Gene Symbol: IL-4R
• Gene ID (NCBI): 3566
• RRID: AB_2882364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.