IRS2 Polyclonal antibody, PBS Only

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• IRS2 Polyclonal antibody, PBS Only (20702-1-PBS)

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  WB analysis using 20702-1-AP (same clone as 20702-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20702-1-AP (IRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

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  WB analysis using 20702-1-AP (same clone as 20702-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20702-1-AP (IRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20702-1-AP (same clone as 20702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20702-1-AP (IRS2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 20702-1-AP (same clone as 20702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20702-1-AP (IRS2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

IRS2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 20702-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Insulin receptor substrate 2, IRS 2, IRS-2

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Product Information

20702-1-PBS targets IRS2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: IRS2
• Calculated Molecular Weight: 137 kDa
• Observed Molecular Weight: 150-185 kDa
• GenBank Accession Number: NM_003749
• Gene Symbol: IRS2
• Gene ID (NCBI): 8660
• RRID: AB_2918067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y4H2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The family of INS receptor substrates (IRS) consists of four proteins (IRS-1-IRS-4), which were initially characterized as typical cytosolic adaptor proteins involved in INS receptor (IR) and INS-like growth factor I receptor (IGF-IR) signaling. Impaired glucose tolerance and INS resistance, and thus type 2 diabetes were demonstrated in Irs2 knockout mice. In vivo data also indicated that he regulation of beta-cell mass involves IRS2, while IRS1 is only required for proper INS production in beta-cells. IRS2, like IRS1, has a predicted molecular weight of 137 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS-PAGE as a band of approximately 150-185 kDa.