ChromoTek ISG15-Trap Agarose

Validation Data Gallery

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  ISG15-Trap agarose (MG-3001A) was used to immunoprecipitate endogenous ISG15 and ISGylated proteins from untreated human HEPG2 cells (baseline) and interferon-β treated HepG2 cells (induced). Lysis was achieved with RIPA Buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-ISG15 polyclonal antibody (15981-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous ISG15 and ISGylated proteins with low background.

• 

  ISG15-Trap agarose (MG-3001A) was used to immunoprecipitate endogenous ISG15 and ISGylated proteins from untreated human HEPG2 cells (baseline) and interferon-β treated HepG2 cells (induced). Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-ISG15 polyclonal antibody (15981-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous ISG15 and ISGylated proteins with low background.

The ChromoTek ISG15-Trap Agarose consists of an anti-ISG15 VHH (Nanobody), which is coupled to agarose beads. It can be used for the immunoprecipitation of ISG15 tagged proteins from cell extracts of various organisms.

Cat No. MG-3001A

Reactivity

ISG15

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Agarose

Synonyms

Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein (IP17), Ubiquitin cross-reactive protein (hUCRP), Ubiquitin-like protein ISG15

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Product Information

The ChromoTek ISG15-Trap Agarose consists of an anti-ISG15 VHH (Nanobody), which is coupled to agarose beads. It can be used for the immunoprecipitation of ISG15 tagged proteins from cell extracts of various organisms.

• Description: Ready to use VHH coupled Agarose Beads for Immunoprecipitation of ISG15.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • Stable in harsh buffer conditions;

  • Suitable for downstream mass spec analysis;

  • High Specificity;

  • Low background

• Applications: IP, Co-IP
• Specificity/Target: /
• Binding Capacity: 5 μg of monovalent ISG15 (~17 kDa) per 25 μL bead slurry
• Bead concentration: 50% Slurry
• Resin Type: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
• Wash Buffer Compatibility: 2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.05% SDS, 1-2 M Urea
• Type: VHH
• Class: Recombinant - Animal free production
• Host: Alpaca
• Affinity: 70 nM
• Compatibility with mass spectrometry: The ISG15-Trap is optimized for on-bead digestion. For the application note, please click here: ; On-bead digest protocol for mass spectrometry
• RRID: AB_3740242
• Storage Buffer: 20% Ethanol
• Storage Condition: Upon arrival store at +4°C / do not freeze!
• Shipping: Ambient Temp

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