CoraLite® Plus 488 Mouse IgG1, κ Isotype Control Recombinant Antibody

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  FC experiment of human PBMCs using CL488-98742

  1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite® Plus 488 Mouse IgG1, κ Isotype Control RecAb (CL488-98742, Clone: 253504A1) (red in histogram) or 0.25 ug CoraLite® Plus 488 Anti-Human CD4 (RPA-T4) (CL488-65143, Clone: RPA-T4) (green in histogram), or unstained (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Cells were not fixed.

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  FC experiment of rat splenocytes using CL488-98742

  1x10^6 rat splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Mouse IgG1, κ Isotype Control RecAb (CL488-98742, Clone: 253504A1) (red in histogram) or 0.25 ug CoraLite® Plus 488 Anti-Rat CD45 (OX1) (CL488-65228, Clone: OX1) (green in histogram), or unstained (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

IgG1, κ Isotype Control Uni-rAb^® Recombinant Antibody for FC

Cat No. CL488-98742

Clone No.253504A1

Host / Isotype

Mouse / IgG1, kappa

Reactivity

n/a

Applications

FC

Synonyms

Mouse IgG1 κ Isotype Control, IgG1 Isotype Control, Mouse IgG1 isotype control

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Tested Applications

• Positive FC detected in: human PBMCs, rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98742 targets IgG1, κ Isotype Control in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Mouse / IgG1, kappa
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: IgG1, κ Isotype Control
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.