Mouse IgG1, λ Isotype Control Recombinant Antibody

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• Mouse IgG1, λ Isotype Control Recombinant Antibody (98741-1-MR)

IgG1, λ Isotype Control Uni-rAb^® Recombinant Antibody for FC

Cat No. 98741-1-MR

Clone No.253505A1

Host / Isotype

Mouse / IgG1

Reactivity

n/a

Applications

FC

Synonyms

Mouse IgG1 λ Isotype Control, IgG1 Isotype Control, Mouse IgG1 isotype control

• 

  FC experiment of human PBMCs using 98741-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Mouse IgG1, λ isotype control RecAb (98741-1-MR, Clone:253505A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or 0.25 ug Anti-Human CD4 (RPA-T4) (65143-1-Ig, Clone: RPA-T4) (green in histogram), or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of rat splenocytes using 98741-1-MR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Mouse IgG1, λ isotype control RecAb (98741-1-MR, Clone:253505A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or 0.25 ug Anti-Rat CD45 (OX1) (65228-1-Ig, Clone: OX1) (green in histogram), or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs, rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98741-1-MR targets IgG1, λ Isotype Control in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: IgG1, λ Isotype Control
• RRID: AB_3746308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

An IgG1, λ isotype control is a critical negative control reagent used in flow cytometry to distinguish specific antibody binding from non-specific background signals. It is an antibody that shares the same host species, immunoglobulin class (IgG1), light chain type (λ), and fluorophore conjugate as the primary antibody used in an experiment, but it lacks specificity for the target antigen.