Rat IgG2a Isotype Control (2A3)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

n/a

Applications

FC

Conjugate

Unconjugated

CloneNo.

2A3

Cat no : 65209-1-Ig

Synonyms

Rat IgG2a Isotype Control, IgG2a

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 65209-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody (red), or 0.25 ug Anti-Mouse CD8a (65069-1-Ig, Clone: 53-6.7) and FITC anti-rat IgG2a Antibody (green) or unstained (blue). Cells were not fixed.

---

Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65209-1-Ig targets IgG2a Isotype Control in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG2a Isotype Control
• RRID: AB_2918471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.