Mouse IgG2b, λ Isotype Control Recombinant Antibody

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• Mouse IgG2b, λ Isotype Control Recombinant Antibody (98739-1-MR)

IgG2b, λ Isotype Control Uni-rAb^® Recombinant Antibody for FC

Cat No. 98739-1-MR

Clone No.253507A1

Host / Isotype

Mouse / IgG2b

Reactivity

n/a

Applications

FC

Synonyms

mouse IgG2b Isotype Control, mouse IgG2b, λ Isotype Control, IgG2b Isotype Control, Mouse IgG2b, mouse IgG2b lambda Isotype

• 

  FC experiment of human PBMCs using 98739-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Mouse IgG2b, λ isotype control RecAb (98739-1-MR, Clone:253507A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or 0.25 ug Anti-Human CD4 (OKT4) (65134-1-Ig, Clone: OKT4) (green in histogram), or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of rat splenocytes using 98739-1-MR

  1x10^6 rat splenocytes cells were surface stained with 0.25 ug Mouse IgG2b, λ isotype control RecAb (98739-1-MR, Clone:253507A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG , or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of THP-1 using 98739-1-MR

  1x10^6 THP-1 cells were surface stained with 0.25 ug Mouse IgG2b, λ isotype control RecAb (98739-1-MR, Clone:253507A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs, THP-1 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98739-1-MR targets IgG2b, λ Isotype Control in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Mouse / IgG2b
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: IgG2b, λ Isotype Control
• RRID: AB_3746306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

An IgG2b, λ isotype control is a negative control reagent essential for flow cytometry experiments. It is designed to match the host species, immunoglobulin class (IgG2b), light chain type (λ), and fluorophore conjugate of the specific primary antibody being used in the study, but crucially, it does not bind to any antigen present on the target cells.