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Mouse IgG2b, λ Isotype Control Recombinant Antibody, PBS Only (98739-1-PBS)

Mouse IgG2b, λ Isotype Control Recombinant Antibody, PBS Only

Name: Mouse IgG2b, λ Isotype Control Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98739-1-PBS-1MG

IgG2b, λ Isotype Control Uni-rAb^® Recombinant Antibody for FC

Cat No. 98739-1-PBS

Clone No.253507A1

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

n/a

Applications

Synonyms

mouse IgG2b Isotype Control, mouse IgG2b, λ Isotype Control, IgG2b Isotype Control, Mouse IgG2b, mouse IgG2b lambda Isotype

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Proteintech Guarantee

Filter: AllFC

FC experiment of human PBMCs using 98739-1-MR (same clone as 98739-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Mouse IgG2b, λ isotype control RecAb (98739-1-MR, Clone:253507A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or 0.25 ug Anti-Human CD4 (OKT4) (65134-1-Ig, Clone: OKT4) (green in histogram), or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98739-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.25 ug Mouse IgG2b, λ isotype control RecAb (98739-1-MR, Clone:253507A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or 0.25 ug Anti-Human CD4 (OKT4) (65134-1-Ig, Clone: OKT4) (green in histogram), or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98739-1-PBS in a different storage buffer formulation.
FC experiment of rat splenocytes using 98739-1-MR (same clone as 98739-1-PBS)
1x10^6 rat splenocytes cells were surface stained with 0.25 ug Mouse IgG2b, λ isotype control RecAb (98739-1-MR, Clone:253507A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG , or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98739-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 rat splenocytes cells were surface stained with 0.25 ug Mouse IgG2b, λ isotype control RecAb (98739-1-MR, Clone:253507A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG , or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98739-1-PBS in a different storage buffer formulation.
FC experiment of THP-1 using 98739-1-MR (same clone as 98739-1-PBS)
1x10^6 THP-1 cells were surface stained with 0.25 ug Mouse IgG2b, λ isotype control RecAb (98739-1-MR, Clone:253507A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98739-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 THP-1 cells were surface stained with 0.25 ug Mouse IgG2b, λ isotype control RecAb (98739-1-MR, Clone:253507A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98739-1-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98739-1-PBS targets IgG2b, λ Isotype Control in FC applications and shows reactivity with n/a samples.

Tested Reactivity	n/a
Host / Isotype	Mouse / IgG2b
Class	Recombinant
Type	Antibody
Immunogen	unknow Predict reactive species
Full Name	IgG2b, λ Isotype Control
Gene Symbol
Gene ID (NCBI)
RRID	AB_3746306
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

An IgG2b, λ isotype control is a negative control reagent essential for flow cytometry experiments. It is designed to match the host species, immunoglobulin class (IgG2b), light chain type (λ), and fluorophore conjugate of the specific primary antibody being used in the study, but crucially, it does not bind to any antigen present on the target cells.