Tested Applications
| Positive FC detected in |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-65243 targets IgM Isotype Control in FC applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Isotype | Mouse / IgM, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | IgM Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2935025 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | N/A |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
The MOPC-104E immunoglobulin is useful as an isotype-matched control for mouse IgM antibodies. B1355S dextran, nigerosyl-alpha (1-3)-nigerose, and nigerosyl-alpha (1-3)-glucose have abilities to bind to MOPC-104E immunoglobulin (PMID: 6165777). This antibody has been quality-tested for flow cytometry as negative control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 IgM Isotype Control antibody CL647-65243 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL647-65243 is the CoraLite® Plus 647-conjugated Mouse IgM Isotype Control (MOPC-104E). It has 1 total citation, published in Cell Reports (IF 7.7). The cited study investigated IgE-dependent anaphylaxis regulated by sphingolipid binding to CD300 family members, placing this citation in the fields of immunology and allergic disease.
| Species | Application | Title |
|---|---|---|
Cell Rep IgE-dependent anaphylaxis is regulated by sphingolipid binding to activating and inhibitory CD300 family members. |
