Mouse Integrin alpha-8 Recombinant monoclonal antibody, PBS Only

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• Mouse Integrin alpha-8 Recombinant monoclonal antibody, PBS Only (87408-1-PBS)

• 

  WB analysis using 87408-1-RR (same clone as 87408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87408-1-RR (Integrin alpha-8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87408-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 87408-1-RR (same clone as 87408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87408-1-RR (Integrin alpha-8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87408-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 87408-1-RR (same clone as 87408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87408-1-RR (Integrin alpha-8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87408-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse lung using 87408-1-RR (same clone as 87408-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Integrin alpha-8 antibody (87408-1-RR, Clone: 252745D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87408-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse lung using 87408-1-RR (same clone as 87408-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Integrin alpha-8 antibody (87408-1-RR, Clone: 252745D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87408-1-PBS in a different storage buffer formulation.

• 

  Affinity of 87408-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87408-1-RR against Mouse Integrin alpha 8 were performed. The affinity constant is 2.24 nM.

Integrin alpha-8 Uni-rAb^® Recombinant Antibody for WB, IF-P, Indirect ELISA

Cat No. 87408-1-PBS

Clone No.252745D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

Integrin alpha-8 heavy chain, Integrin alpha-8 light chain, Itga8

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Product Information

87408-1-PBS targets Integrin alpha-8 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10469

  Product name: Recombinant mouse ITA8 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011446 of

  Sequence: \

• Full Name: integrin alpha 8
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 140 kDa
• Gene Symbol: Itga8
• Gene ID (NCBI): 241226
• RRID: AB_3745523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: A2ARA8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).