Integrin alpha-8 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• Integrin alpha-8 Recombinant monoclonal antibody (87408-1-RR)

• 

  WB analysis using 87408-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87408-1-RR (Integrin alpha-8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87408-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87408-1-RR (Integrin alpha-8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87408-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87408-1-RR (Integrin alpha-8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse lung using 87408-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Integrin alpha-8 antibody (87408-1-RR, Clone: 252745D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 87408-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Integrin alpha-8 antibody (87408-1-RR, Clone: 252745D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity of 87408-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87408-1-RR against Mouse Integrin alpha 8 were performed. The affinity constant is 2.24 nM.

Integrin alpha-8 Uni-rAb^® Recombinant Antibody for WB, IF-P, ELISA

Cat No. 87408-1-RR

Clone No.252745D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

Integrin alpha-8 heavy chain, Integrin alpha-8 light chain, Itga8

---

Tested Applications

• Positive WB detected in: mouse spleen tissue, human kidney tissue, mouse lung tissue, mouse kidney tissue, rat lung tissue, rat spleen tissue, rat kidney tissue
• Positive IF-P detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87408-1-RR targets Integrin alpha-8 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10469

  Product name: Recombinant mouse ITA8 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011446 of

  Sequence: \

• Full Name: integrin alpha 8
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 140 kDa
• Gene Symbol: Itga8
• Gene ID (NCBI): 241226
• RRID: AB_3745523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: A2ARA8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).