Integrin beta-5 Recombinant antibody
Integrin beta-5 Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240401G11
Cat no : 83514-3-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HT-29 cells, NIH-H1299 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83514-3-RR is Integrin beta-5 Fusion Protein expressed in E. coli.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Integrin beta-5 fusion protein Ag29783 |
Full Name | integrin, beta 5 |
Calculated Molecular Weight | 88 kDa |
Observed Molecular Weight | 88-100 kDa |
GenBank Accession Number | BC006541 |
Gene Symbol | Integrin beta 5 |
Gene ID (NCBI) | 3693 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ITGB5, also known as Integrin beta-5. It is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869). The molecular weight of ITGB5 is 88 kDa, and the glycosylation modification also exists.
Protocols
Product Specific Protocols | |
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WB protocol for Integrin beta-5 antibody 83514-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |