JAML Polyclonal antibody, PBS Only

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• JAML Polyclonal antibody, PBS Only (21302-1-PBS)

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  WB analysis using 21302-1-AP (same clone as 21302-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21302-1-AP (JAML antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21302-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 21302-1-AP (same clone as 21302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 21302-1-AP (JAML antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21302-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 21302-1-AP (same clone as 21302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 21302-1-AP (JAML antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21302-1-PBS in a different storage buffer formulation.

JAML Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 21302-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Gm638, Dendritic cell-specific protein CREA7-1, CREA7 4, CREA7 1, AMICA1

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Product Information

21302-1-PBS targets JAML in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15900

  Product name: Recombinant human AMICA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 40-275 aa of BC100797

  Sequence: VPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVSPEEPRTLVTPAALRPLVLGGNQLVIIVGIVCATILLLPVLILIVKKTCGNKSSVNSTVLVKN

• Full Name: adhesion molecule, interacts with CXADR antigen 1
• Calculated Molecular Weight: 394 aa, 44 kDa
• Observed Molecular Weight: 44 kDa, 50-70 kDa
• GenBank Accession Number: BC100797
• Gene Symbol: JAML
• Gene ID (NCBI): 120425
• RRID: AB_10733103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86YT9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Junctional adhesion molecules (JAM-A, JAM-B, and JAM-C) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites (PMID: 19064666). JAML (junctional adhesion molecule-like), also known as AMICA1, is related to JAM proteins. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML is expressed on leukocytes and promotes their adhesion to endothelial cells (PMID: 12869515). JAML can regulate neutrophil transepithelial migration via binding interactions with epithelial coxsackie and adenovirus receptor (CAR) (PMID: 15800062). The full-length human JAML protein has a calculated molecular weight of 44 kDa. This antibody raised against 40-275 aa of human JAML protein detects bands with molecular wight of 44 kDa, and 50-70 kDa which probably represent different glycoforms of the protein (PMID: 12869515; 24621992; 15800062).