JMJD6 Polyclonal antibody

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• JMJD6 Polyclonal antibody (16476-1-AP)

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• KD/KO Validated

JMJD6 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 16476-1-AP Copied!

Publications (16)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, EC:1.14.11.-, JmjC domain-containing protein 6, Jumonji domain-containing protein 6, KIAA0585

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  WB analysis of mouse liver using 16476-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16476-1-AP (JMJD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of K-562 using 16476-1-AP

  IP result of anti-JMJD6 (IP:16476-1-AP, 4ug; Detection:16476-1-AP 1:500) with K-562 cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of A549 using 16476-1-AP

  1x10^6 A549 cells were intracellularly stained with 0.4 ug JMJD6 Polyclonal antibody (16476-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse heart tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16476-1-AP targets JMJD6 in WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9590

  Product name: Recombinant human JMJD6 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-403 aa of BC066654

  Sequence: MNHKSKKRIREAKRSARPELKDSLDWTRHNYYESFSLSPAAVADNVERADALQLSVEEFVERYERPYKPVVLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEYMESTRDDSPLYIFDSSYGEHPKRRKLLEDYKVPKFFTDDLFQYAGEKRRPPYRWFVMGPPRSGTGIHIDPLGTSAWNALVQGHKRWCLFPTSTPRELIKVTRDEGGNQQDEAITWFNVIYPRTQLPTWPPEFKPLEILQKPGETVFVPGGWWHVVLNLDTTIAITQNFASSTNFPVVWHKTVRGRPKLSRKWYRILKQEHPELAVLADSVDLQESTGIASDSSSDSSSSSSSSSSDSDSECESGSEGDGTVHRRKKRRTCSMVGNGDTTSQDDCVSKERSSSR

• Full Name: jumonji domain containing 6
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC066654
• Gene Symbol: JMJD6
• Gene ID (NCBI): 23210
• RRID: AB_2265134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6NYC1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

JMJD6 affinity purification antibody (Cat# 16476-1-AP) has 16 citations published in journals including Molecular Cell, Nucleic Acids Research, Theranostics, Cell Reports, Journal of Medicinal Chemistry, Acta Pharmacologica Sinica, and PLoS Pathogens. Associated research fields encompass epigenetics, transcription regulation, ferroptosis, cancer biology (liver, prostate, breast, glioblastoma), cardiac hypertrophy, antiviral signaling, and tumor microenvironment analysis.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IP,ChIP	Mol Cell; JMJD6 Licenses ERα-Dependent Enhancer and Coding Gene Activation by Modulating the Recruitment of the CARM1/MED12 Co-activator Complex.; Authors - Wei-Wei Gao ; KD Validated
human	IP,ChIP	Nucleic Acids Res; ARGLU1 enhances promoter-proximal pausing of RNA polymerase II and stimulates DNA damage repair; Authors - Scott Bachus
human	WB	Theranostics; PRMT6-mediated ADMA promotes p62 phase separation to form a negative feedback loop in ferroptosis; Authors - Lifeng Feng
rat,human	WB,IP	Acta Pharmacol Sin; JMJD6 protects against isoproterenol-induced cardiac hypertrophy via inhibition of NF-κB activation by demethylating R149 of the p65 subunit; Authors - Zhen Guo
human	ChIP	J Transl Med; JMJD6-BRD4 complex stimulates lncRNA HOTAIR transcription by binding to the promoter region of HOTAIR and induces radioresistance in liver cancer stem cells; Authors - Ruifeng Pei
human	WB	Chem Sci; A constitutional isomer selective chemical proteomic strategy for system-wide profiling of protein lysine 5-hydroxylation; Authors - Yi-Cheng Sin