JMJD6 Polyclonal antibody

• Featured Product
• KD/KO Validated

JMJD6 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 16476-1-AP

Synonyms

Lysyl-hydroxylase JMJD6, KIAA0585, Jumonji domain-containing protein 6, JmjC domain-containing protein 6, EC:1.14.11.-

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Validation Data Gallery

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  WB analysis of mouse liver using 16476-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16476-1-AP (JMJD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16476-1-AP

  IP result of anti-JMJD6 (IP:16476-1-AP, 4ug; Detection:16476-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of mouse heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of A549 using 16476-1-AP

  1x10^6 A549 cells were intracellularly stained with 0.4 ug JMJD6 Polyclonal antibody (16476-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse heart tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 4 publications below

Product Information

16476-1-AP targets JMJD6 in WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JMJD6 fusion protein Ag9590
• Full Name: jumonji domain containing 6
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC066654
• Gene Symbol: JMJD6
• Gene ID (NCBI): 23210
• RRID: AB_2265134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IP,ChIP	Mol Cell; JMJD6 Licenses ERα-Dependent Enhancer and Coding Gene Activation by Modulating the Recruitment of the CARM1/MED12 Co-activator Complex.; Authors - Wei-Wei Gao ; KD Validated
human,mouse	IHC	Front Immunol; Single-cell protein activity analysis reveals a novel subpopulation of chondrocytes and the corresponding key master regulator proteins associated with anti-senescence and OA progression; Authors - Zhao Guang
human	WB	PLoS Pathog; JMJD6 negatively regulates cytosolic RNA induced antiviral signaling by recruiting RNF5 to promote activated IRF3 K48 ubiquitination.; Authors - Wei Zhang ; KO Validated
human	WB	J Med Chem; Discovery of Small-Molecule Degraders of the CDK9-Cyclin T1 Complex for Targeting Transcriptional Addiction in Prostate Cancer; Authors - Jiacheng Li
human	ChIP	Cell Biol Int; c-Jun-mediated JMJD6 restoration enhances resistance of liver cancer to radiotherapy through the IL-4-activated ERK pathway; Authors - Yong Liu
human,rat	WB,IP	Acta Pharmacol Sin; JMJD6 protects against isoproterenol-induced cardiac hypertrophy via inhibition of NF-κB activation by demethylating R149 of the p65 subunit; Authors - Zhen Guo