KARS Polyclonal antibody, PBS Only

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• KARS Polyclonal antibody, PBS Only (14951-1-PBS)

• 

  WB analysis using 14951-1-AP (same clone as 14951-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 14951-1-AP (same clone as 14951-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 14951-1-AP (same clone as 14951-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

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  IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 14951-1-AP (same clone as 14951-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KARS antibody (14951-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

KARS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14951-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Lysyl-tRNA synthetase, Lysine--tRNA ligase, KIAA0070, KARS1, EC:2.7.7.-

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Product Information

14951-1-PBS targets KARS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6778

  Product name: Recombinant human KARS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 246-597 aa of BC004132

  Sequence: IRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

• Full Name: lysyl-tRNA synthetase
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC004132
• Gene Symbol: KARS
• Gene ID (NCBI): 3735
• RRID: AB_2128158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15046
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KARS (lysyl-tRNA synthetase) is a gene that encodes an enzyme critical for protein synthesis, specifically responsible for attaching the amino acid lysine to its corresponding transfer RNA (tRNA) in both the cytoplasm and mitochondria. This enzyme plays a crucial role in the process of aminoacylation, which is essential for accurate protein translation from mRNA sequences. Mutations in the KARS gene have been associated with a variety of neurological and systemic disorders, reflecting the enzyme's importance in cellular function.