KARS Polyclonal antibody, PBS Only

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• KARS Polyclonal antibody, PBS Only (14951-1-PBS)

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• KD/KO Validated

KARS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14951-1-PBS Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:2.7.7.-, KARS1, KIAA0070, Lysine--tRNA ligase, Lysyl-tRNA synthetase

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  WB analysis using 14951-1-AP (same clone as 14951-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 14951-1-AP (same clone as 14951-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human colon using 14951-1-AP (same clone as 14951-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14951-1-AP (KARS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14951-1-AP (KARS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14951-1-AP (KARS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse adrenal gland using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 14951-1-AP (KARS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat adrenal gland using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 14951-1-AP (KARS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14951-1-AP (KARS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 14951-1-AP (same clone as 14951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14951-1-AP (KARS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 14951-1-AP (same clone as 14951-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KARS antibody (14951-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

14951-1-PBS targets KARS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6778

  Product name: Recombinant human KARS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 246-597 aa of BC004132

  Sequence: IRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

• Full Name: lysyl-tRNA synthetase
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC004132
• Gene Symbol: KARS
• Gene ID (NCBI): 3735
• RRID: AB_2128158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15046
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KARS (lysyl-tRNA synthetase) is a gene that encodes an enzyme critical for protein synthesis, specifically responsible for attaching the amino acid lysine to its corresponding transfer RNA (tRNA) in both the cytoplasm and mitochondria. This enzyme plays a crucial role in the process of aminoacylation, which is essential for accurate protein translation from mRNA sequences. Mutations in the KARS gene have been associated with a variety of neurological and systemic disorders, reflecting the enzyme's importance in cellular function.